MICROINJECTION OF COLCHICINE INTO SEA URCHIN EGGS
نویسندگان
چکیده
منابع مشابه
Cleavage of Sea-urchin Eggs in Colchicine
INTRODUCTION We have recently put forward a theory linking mitosis with cleavage, in which we suggest that cleavage is caused by an expansion of the cell cortex which starts at either pole of the cell and later moves round to the region of the furrow (Mitchison, 1952). There is evidence that this expansion is brought about by substances diffusing from the two groups of daughter chromosomes whic...
متن کاملTHE MECHANISM OF ACTION OF COLCHICINE Colchicine Binding to Sea Urchin Eggs and the Mitotic Apparatus
Colchicine forms a complex in vivo with a protein present in fertilized or unfertilized sea urchin eggs; similar binding was obtained in vitro with the soluble fraction from egg homogenates. Kinetic parameters and binding equilibrium constant were essentially the same in vivo and in vitro. The binding site protein was shown to have a sedimentation constant of 6S by zone centrifugation. The prot...
متن کاملMicroinjection of fluorescent tubulin into dividing sea urchin cells
To follow the dynamics of microtubule (MT) assembly and disassembly during mitosis in living cells, tubulin has been covalently modified with the fluorochrome 5-(4,6-dichlorotriazin-2-yl)aminofluorescein and microinjected into fertilized eggs of the sea urchin Lytechinus variegatus. The changing distribution of the fluorescent protein probe is visualized in a fluorescence microscope coupled to ...
متن کاملProperties of tubulin in unfertilized sea urchin eggs. Quantitation and characterization by the colchicine-binding reaction
The colchicine-binding assay was used to quantitate the tubulin concentration in unfertilized Strongylocentrotus purpuratus eggs and to characterize pharmacological properties of this tubulin. Specificity of colchicine binding to tubulin was demonstrated by apparent first-order decay colchicine-binding activity with stabilization by vinblastine sulfate, time and temperature dependence of the re...
متن کاملAction of Colcemid in Sea Urchin Eggs
The effects of Colcemid, the deacetyl-N-methyl derivative of colchicine, on the eggs of Arbacia punctulata were investigated. Colcemid in concentrations of 2.7 x 10(-5)M or greater blocks syngamy (the fusion of the pronuclei) in these eggs. Although a tenfold decrease in concentration of Colcemid usually permits the pronuclei to fuse, the subsequent division is blocked. In the sea urchin egg, t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1975
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1975.00111.x